Coding
Part:BBa_K2476012:Design
Designed by: Vivian Hu Group: iGEM17_Pittsburgh (2017-10-27)
WT CheY + Linker/Dronpa/Linker at 60-61
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1076
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimized for Escherichia coli K12 and checked for BioBrick restriction sites using IDT Codon Optimization Tool.
Chemotaxis protein CheY with flexible serine-glycine linkers and a Dronpa monomer attached between M60-P61.
Source
Chemotaxis protein CheY found in Escherichia coli (strain K12) with flexible serine-glycine linkers and a pdDronpa1.2 monomer (http://science.sciencemag.org/content/355/6327/836.full) attached between M60-P61. Synthesized by IDT.